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. Author manuscript; available in PMC: 2008 Aug 22.

Published in final edited form as: ACS Chem Biol. 2007 Oct 19;2(10):674–684. doi: 10.1021/cb7001472

Effects the PLC inhibitor, U73122, and the PI3 kinase inhibitor, wortmannin, on ligand-stimulated release of Ca²⁺ from intracellular stores. Cells were pre-incubated for 5 min in the absence or presence of either 1 μM U73122 or 200 nM wortmannin prior to addition of Y16-DNP3 (a), Y46-DNP (b), or 1 μg/mL DNP-BSA (c) as in Fig. 5.

Effects the PLC inhibitor, U73122, and the PI3 kinase inhibitor, wortmannin, on ligand-stimulated release of Ca²⁺ from intracellular stores. Cells were pre-incubated for 5 min in the absence or presence of either 1 μM U73122 or 200 nM wortmannin prior to addition of Y16-DNP3 (a), Y46-DNP (b), or 1 μg/mL DNP-BSA (c) as in Fig. 5.