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. Author manuscript; available in PMC: 2008 Aug 22.
Published in final edited form as: Bioconjug Chem. 2006;17(4):920–927. doi: 10.1021/bc060044i

Figure 2. Dose-dependent cellular internalization of QD-CPP assemblies.

Figure 2

HEK 293T/17 cells were incubated with 510-nm QD-CPP assemblies (QD:CPP ratio 1:50) at QD concentrations of: 250 nM (A), 60 nM (B), 15 nM (C) or with 250 nM QDs without CPP (D); cells were also stained with DAPI. Overlay of phase contrast, QD fluorescence (green), and DAPI fluorescence (blue) show the dose-dependent nature of the QD-CPP delivery. Images also show that QD staining is non-nuclear, punctate fluorescence located in the cytoplasm. Negligible cell-surface associated fluorescence was observed. Arrows indicate regions of QD-CPP internalization at lower concentrations. Scale bar is 10 μm.