Table 1. Sequences of primers used to amplify the coding regions of the RS1 gene.
| Exon | Primers (5′-3′) | Product size (bp) | Ta (°C) |
|---|---|---|---|
| 1 |
F: CTCAGCCAAAGACCTAAGAAC |
216 |
58 |
| R: GTATGCAATGAATGTCAATGG |
|||
| 2 |
F: GTGATGCTGTTGGATTTCTC |
176 |
56 |
| R: CAAAGTGATAGTCCTCTATG |
|||
| 3 |
F: CGATGCATAAGGACTGAGTGTGATC |
377 |
50 |
| R: GCATTAACATAGGCTTAC TAATAG |
|||
| 4 |
F: CGTGAGTAGTGAACCGTTGAAGAC |
381 |
50 |
| R: ACGCTGGTAGAGAGGCCTAT |
|||
| 5 |
F: GCAAGTTAAGTATAACGGAAGCTG G |
508 |
50 |
| R: GGAAAGCGCAGATGATCCACTGTG |
|||
| 6 |
F: GCAAACTGCTTTAACTAC TTCC |
427 |
50 |
| R: CCAGCACTGCAGTTACAATTGC |
Shown are primer sequences, annealing temperatures, and anticipated size of the amplified product for the different fragments of RS1 studied. The following abbreviations were used: base pair (bp) and annealing temperature (Ta). The template sequence is available at NCBI, GenBank accession number: RS1: NM_000330.