Table 1.
Fluorescence maxima of NATA and OmpA mutants unfolded in urea, folded in OG, and folded in DMPC, and ratios of quenched to unquenched emission intensities (F/F0) and lifetimes (τ/τ0) for full-length OmpA mutants in DMPC and DMPC/6,7-DiBr. Lifetime ratios are based on amplitude-weighted average lifetimes from tri-exponential decay fits.
| Fluorescence Maxima (nm) | Quenching | ||||
|---|---|---|---|---|---|
| Urea | OG | DMPC | F/F0 | τ/τ0 | |
| NATA | 352 | 352 | 352 | 1 | 1 |
| W7 | 346 | 322 | 318 | 0.4 | 0.6 |
| W7t | 346 | 324 | 318 | ----- | ----- |
| W15 | 346 | 328 | 326 | 0.4 | 0.6 |
| W15t | 346 | 328 | 326 | ----- | ----- |
| W57 | 346 | 324 | 325 | 0.5 | 0.7 |
| W57t | 346 | 326 | 325 | ----- | ----- |
| W102 | 346 | 326 | 324 | 0.8 | 1 |
| W102t | 346 | 326 | 324 | ----- | ----- |
| W143 | 346 | 326 | 326 | 0.4 | 0.7 |
| W143t | 346 | 326 | 327 | ----- | ----- |