Figure 5.
Location of endogenous parafibromin and actinin in C2C12 myoblasts or C2C12 differentiated into myotubes. C2C12 cells were analyzed by immunofluorescence with anti-parafibromin (GRAPE) antibody and anti-actinin antibody before (A, B, C) or after differentiation (D, E, F), and secondary antibody conjugated to Texas Red (to detect parafibromin) or fluorescein isothiocyanate (to detect actinin), followed by DAPI staining (A, D). Note that in panel E, along with myotubes there are some undifferentiated myoblasts that retain nuclear parafibromin staining (arrows). Magnification, 60× (A, B, C), or 10× (D, E, F).