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DNA helicase assays using full-length wild-type RecD2 and the pin-less mutant. The substrate used was a 20 base-pair duplex with a 12 base 5′-tail. The top panel shows a time course with wild-type and pin-less mutant. The panel below shows the end products of a 16 min assay at increasing concentrations of the protein. A boiled control lane is also shown.
