Table 1.
Crystallographic statistics
D. radiodurans RecD2 | Native | Hg | W | Se |
---|---|---|---|---|
Resolution (Å) | 20–2.2 | 20–3.2 | 20–2.8 | 20–3.0 |
Completeness (%) | 98.0 (98.0) | 97.7 (90.0) | 97.1 (79.2) | 97.4 (83.8) |
Rsymm (%) | 3.8 (32.1) | 6.7 (26.3) | 2.4 (16.0) | 74.1 (21.6) |
Rderiv (%) | — | 31.4 | 14.8 | 31.8 |
No. of sites | — | 1 | 1 | |
Phasing power | — | 0.3 | 0.9 | |
Mean figure of merit | 0.35 | |||
E. coli RecBCD | IodoDNA | IodoDNA (low dose) | IodoDNA (high dose) | |
Resolution (Å) | 30–3.6 | 30–4.0 | 30–4.0 | |
Completeness (%) | 96.7 (97.5) | 99.6 (100) | 93.1 (63.3) | |
Rsymm (%) | 7.5 (35.1) | 10.0 (23.8) | 11.0 (29.1) | |
Final models | D. radiodurans RecD2 | E. coli RecBCD | ||
R-factor (%) | 23.1 | 24.6 | ||
Rfree (%) | 28.6 | 30.0 | ||
r.m.s.d. bond length (Å) | 0.009 | 0.011 | ||
r.m.s.d. bond angles (deg) | 1.3 | 1.7 | ||
Figures in parentheses indicate highest resolution shell. |