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. 1999 Mar;10(3):609–626. doi: 10.1091/mbc.10.3.609

Figure 1.

Figure 1

(A and B) Phenotype of class II Kar− zygotes. Shown are examples of wild-type (A) and class II Kar− (B) zygotes, respectively. Zygotes from filter matings between wild-type strains (MS1554 × MS23) or between kar7-1039 strains (MS3259 × MS3539) were analyzed by microscopy. Each image shows the nucleus by DAPI fluorescence and the zygote morphology by DIC. (C) Temperature-sensitive defect of kar7-1039. Streaks of wild-type (MS1554), kar7-1039 (MS3259), and sec71Δ (MS3910) strains were incubated at 30°C (left panel) or 37°C (right panel).