Table 1.
Yeast strains used in this study
| Strain | Genotype |
|---|---|
| MS5 | MATa ura3-52 leu2-3 leu2-112 |
| MS10 | MATa ura3-52 leu2-3, leu2-112 ade2-101 |
| MS16 | MATα ade2-101 trp1-Δ1 |
| MS22 | MATa ade2-101 trp1-Δ1 lys2-801 |
| MS23 | MATα ade2-101 trp1-Δ1 lys2-801 |
| MS31 | MATα trp1-Δ1 |
| MS52 | MATα ura3-52 leu2-3 leu2-112 trp1-Δ1 |
| MS1111 | MATa ura3-52 leu2-3 leu2-112 ade2-101 kar2-1 |
| MS1554 | MATa ura3-52 leu2-3, leu2-112 ade2-101 his3-Δ200 |
| MS2705 | MATΔ∷LEU2 ura3-52 leu2-3, leu2-112 trp1-Δ1 kar8-1333 [YCpMATα] |
| MS2706 | MATΔ∷LEU2 ura3-52 leu2-3, leu2-112 trp1-Δ1 kar8-1333 |
| MS3259 | MATa ura3-52 leu2-3, leu2-112 ade2-101 his3-Δ200 kar7-1039 |
| MS3260 | MATa ura3-52 leu2-3 leu2-112 his3-Δ200 ade2-101 kar8-1333 |
| MS3534 | MATa ura3-52 leu2-3, leu2-112 ade2-101 his3-Δ200 lys2-801 kar5-1162 |
| MS3536 | MATα ura3-52 lys2-801 his3-Δ200 ade2-101 leu2-3 leu2-112 kar8-1333 |
| MS3537 | MATα ura3-52 ade2-101 his3-Δ200 lys2-801 kar5-486 |
| MS3538 | MATα ura3-52 ade2-101 his3-Δ200 lys2-801 kar5-1162 |
| MS3539 | MATα ura3-52 ade2-101 his3-Δ200 lys2-801 kar7-1039 |
| MS3577 | MATα ura3-52 leu2-3, leu2-112 trp1-Δ1 kar5-Δ2∷LEU2 |
| MS3823 | MATa ura3-52 ade2-101 kar7-1039 |
| MS3826 | MATα ura3-52 leu2-3, leu2-112 ade2-101 kar7-1039 |
| MS3856 | MATα ura3-52 leu2-3, leu2-112 trp1-Δ1 ρo cyh2 |
| MS3908 | MATa ura3-52 leu2-3, leu2-112 ade2-101 his3-Δ200 SEC71∷YIp-LEU2 |
| MS3910 | MATa ura3-52 leu2-3, leu2-112 ade2-101 his3-Δ200 sec71-Δ1∷URA3 |
| MS3911 | MATα ura3-52 ade2-101 his3-Δ200 lys2-801 sec71-Δ1∷URA3 |
| MS3927 | MATα ura3-52 leu2-3, leu2-112 trp1-Δ1 sec71-Δ1∷URA3ρo cyh2 |
| MS3928 | MATa ura3-52 leu2-3 leu2-112 kar8-1333 |
| MS3986 | MATa ura3-52 leu2-3, leu2-112 ade2-101 his3-Δ200 lys2-801 kar5-Δ2∷LEU2 (pRS426) |
| MS3987 | MATa ura3-52 leu2-3, leu2-112 his3-Δ200 kar5-Δ2∷LEU2 (pMR3142) |
| MS3989 | MATa ura3-52 leu2-3, leu2-112 ade2-101 lys2-801 kar5-1039 (pMR3142) |
| MS3991 | MATa ura3-52 leu2-3, leu2-112 ade2-101 lys2-801 kar7-1039 (pMR3142) |
| MS4020 | MATa ura3-52 ade2-101 leu2-3, leu2-112 kar2-1 (pMR3142) |
| MS4021 | MATa ura3-52 leu2-3 kar1-1 (pMR3142) |
| MS4060 | MATα ura3-52 ade2-101 his3-Δ200 lys2-801 kar7-1039 ρo cyh2 |
| MS4076 | MATa ura3-52 leu2-3 leu2-112 kar8-Δ1∷LEU2 |
| MS4326 | MATα ura3-52 leu2-3 leu2-112 his3-Δ200 ade2-101 trp1-Δ1 |
| MS4338 | MATα ura3-52 leu2-3 leu2-112 trp1-Δ1 his3-Δ200 kar8-Δ1∷LEU2 |
| MS4342 | MATa ura3-52 leu2-3 leu2-112 his3-Δ200 kar8-Δ1∷LEU2 |
| MS4359 | MATα ura3-52 leu2-3 leu2-112 ade2-101 his3-Δ200 kar5-Δ1∷URA3 kar8-Δ1∷LEU2 |
| MS4360 | MATa ura3-52 leu2-3 leu2-112 ade2-101 his3-Δ200 trp1-Δ1 kar5-Δ1∷URA3 kar8-Δ1∷LEU2 |
| MY2248a | MATα ura3-52 leu2-3 leu2-112 ade2-101 trp1-Δ1 sec63-1 |
| MY2339 | MATα ura3-52 ade2-101 his4-539 sec61-2 |
| MY2341 | MATa ura3-52 leu2-3, leu2-112 ade2-101 sec61-2 |
| MY2653a | MATα ura3-52 leu2-3 leu2-112 trp1-Δ1 sec63-4 |
| MY2808 | MATa trp1Δ sec63-1 |
| MY3564 | MATα ura3-52 sec63-1 ρo cyh2 |
| MY3594 | MATa ura3-52 ade2-101 his4-539 trp1-Δ1 sec62-1 |
| MY3676 | MATα ura3-52 ade2-101 his4-539 sec61-2 ρo cyh2 |
| MY3678 | MATα ura3-52 leu2-3, leu2-112 his4-539 sec62-1 ρo cyh2 |
| MY3917 | MATα ura3-52 ade2-101 his3-Δ200 lys2-801 trp1-Δ1 sec72-Δ∷HIS3 |
| MY3918 | MATa ura3-52 leu2Δ1 ade2-101 his3-Δ200 sec72-Δ∷HIS3 |
| MY3931 | MATα ura3-52 ade2-101 his3-Δ200 lys2-801 trp1-Δ1 sec72-Δ∷HIS3 ρo cyh2 |
| MY4169a | MATα ura3-52 leu2-Δ1 ade2-101 lys2-801 trp1-Δ1 his3-Δ200 sec63-Δ∷HIS3 (SEC63-URA 2μ) |
| MLY1600a | MATα ura3-52 leu2-3, leu2-112 pep4-Δ∷URA3 |
| MLY1601a | MATa ura3-52 leu2-3, leu2-112 pep4-Δ∷URA3 gls1-1 |
| MLY1651a | MATα ura3-52 leu2-3, leu2-112 pep4-Δ∷URA3 sec63-1 |
| MLY1652a | MATα ura3-52 leu2-3, leu2-112 pep4-Δ∷URA3 gls1-1 sec63-1 |
| MLY1889a | MATa ura3-52 leu2-3, leu2-112 pep4-Δ∷URA3 gls1-1 sec71-Δ |
| MLY1890a | MATα ura3-52 leu2-3, leu2-112 pep4-Δ∷URA3 sec71-Δ |
| MLY1891a | MATa ura3-52 leu2-3, leu2-112 pep4-Δ∷URA3 gls1-1 sec72-Δ |
| MLY1892a | MATα ura3-52 leu2-3, leu2-112 pep4Δ∷URA3 sec72-Δ |
| DNY65b | MATa sec63-201 ura3-Δ99 leu2-Δ99 his3-Δ200 leu2-Δ1 trp1-Δ99 ade2-101 (pDN106) |
| DNY66b | MATα sec63-201 ura3-Δ99 leu2-Δ99 his3-Δ200 leu2-Δ1 trp1-Δ99 ade2-101 (pDN106) |
a
Obtained from R. Schekman laboratory.
b
Obtained from Davis Ng (P. Walter Laboratory).