Table 3.

Quantitative mating experiments of various protein translocation-defective mutants

	Diploids (%)	C:D ratio
Wild type
23°C	49	0.0009
30°C	49	0.0008
35°C	21	0.0003
sec61-2
23°C	27	0.0018
30°C	7	0.0018
35°C	2	0.0017
sec62-1
23°C	38	0.0038
30°C	6	0.076
35°C	6	0.0083
sec63-1
23°C	5	0.22
30°C	3	0.028
35°C	0.2	0.011
kar7-1039
23°C	9	0.02
30°C	3	0.52
35°C	0.1	1.2
sec71Δ
23°C	5	0.19
30°C	2	0.25
35°C	1	0.53
sec72Δ
23°C	24	0.0013
30°C	15	0.0041
35°C	11	0.001

Quantitative mating experiments performed at 23, 30, and 35°C. Percent diploid formation represents the number of diploids measured on appropriate selection plates divided by the number of viable cells measured on YPD plates × 100. C:D ratio reports the number of cytoductants measured on YPG cycloheximide plates divided by the number of diploids formed. The matings were as follows: wild type (MS1554 × MS3856); sec61-2 (MY2341 × MY3676); sec62-1 (MY3594 × MY3678); sec63-1 (MY2808 × MY3564); kar7-1039 (MS3259 × MS4060); sec71Δ (MS3910 × MS3927); and sec72Δ (MY3918 × MY3931). In general, matings against a wild-type strain gave a greater mating efficiency than mating involving the mutants themselves and a C:D ratio comparable to the wild-type × wild-type mating. However, sec63-1, kar7-1039, and sec71Δ strains resulted in a C:D ratio of 10⁻²–10⁻³ even when these mutants were mated against wild type.