Table 4.

Nuclear fusion defect of protein translocation mutants by microscopic analysis of zygotes

Mating mixtures were stained with DAPI, and the phenotypes of the zygotes were observed by microscopy. Numbers represent percentages of wild-type (wt) or karyogamy-defective class II (Kar⁻) zygotes. At least 100 zygotes were analyzed. The sec61-2, sec62-1, and sec63-1 strains were pregrown at 23°C. Filter matings were performed for 2.5 h at 30°C except for sec63-1, which was performed for 4 h at 23°C. The matings were as follows: wild type × wild type (MS1554 × MS23); sec61-2 × sec61-2 (MY2341 × MY2339); sec62-1 × sec62-1 (MY3594 × MY3678); sec63-1 × sec63-1 (MY2808 × MY3564); kar7-1039 × kar7-1039 (MS3823 × MS3539); sec71Δ × sec71Δ (MS3910 × MS3911); and sec72Δ × sec72Δ (MY3918 × MY3917).