Table 5.

sec71 and kar5 mutants show synthetic bilateral nuclear fusion defects

Mating mixtures were stained with DAPI, and the phenotypes of the zygotes were observed by microscopy. Numbers represent percentages of wild-type (wt) or karyogamy-defective class II (Kar⁻) zygotes. At least 100 zygotes were analyzed. The matings were as follows: wild type × wild type (MS1554 × MS23); sec71Δ × wild type (MS3910 × MS16); kar5-1162 × wild type (MS3534 × MS23); sec71Δ × sec71Δ (MS3910 × MS3911); kar5-1162 × kar5-1162 (MS3534 × MS3538); kar7Δ × kar5Δ (MS3910 × MS3577); kar7Δ × kar5-486 (MS3910 × MS3537); kar7Δ × kar5-1162 (MS3910 × MS3538); and kar7-1039 × kar5-1162 (MS3823 × MS3538). 

^* Data taken from Beh et al. (1997).