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. 1999 Mar;10(3):609–626. doi: 10.1091/mbc.10.3.609

Table 6.

Nuclear fusion defect of kar8-1333 and jem1/kar8Δ by microscopic analysis of zygotes

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Mating mixtures were stained with DAPI, and the phenotypes of the zygotes were observed by microscopy. Numbers represent percentages of wild-type (wt) or karyogamy-defective class II (Kar−) F zygotes. At least 200 zygotes were analyzed. The matings were as follows: wild type × wild type (MS1554 × MS52); wild type × kar8-1333 (MS1554 × MS3536); kar8-1333 × kar8-1333 (MS3260 × MS3536); jem1Δ × jem1Δ (MS4342 × MS4338); and jem1Δ × kar8-1333 (MS4342 × MS3536).