Table 7.

Dosage suppression of kar2, kar8, and sec63 mutants

Mating mixtures were stained with DAPI, and the phenotypes of the zygotes were observed by microscopy. Numbers represent percentages of wild-type (wt) or karyogamy-defective class II (Kar⁻) zygotes. At least 200 zygotes were analyzed. The matings were as follows: wild type × wild type (MS1554 × MS52) transformed with a vector control (pRS426); kar2-1 × wild type (MS1111 × MS31) in which MS1111 was transformed with vector (pRS426), JEM1-CEN (pMR3260), JEM1 2μ (pMR 3270), SEC63-CEN (pCen63), SEC63 2μ (pDF15), or SCJ1 2μ (pPS720); kar8-1333 × kar8-1333 (MS 3928 × MS3536) transformed with vector (pRS426), SEC63-CEN (pCen63), SEC63 2μ (pDF15), or SCJ1 2μ (pPS720); and sec63-201 × sec63-201 (DNY65 × DNY66) transformed with vector (pRS425), JEM1-CEN (pMR 3369), JEM1 2μ (pMR3352), or SEC63 2μ (pDF14). In the kar2-1 × wild-type cross, only the kar2-1 parent was transformed with the indicated plasmids. In the case of the kar8-1333 × kar8-1333 and sec63-201 × sec63-201 matings, both parents were transformed with the indicated plasmids. Data for the table were gathered from several independent experiments.