Table 4.

FFA_u distributions determined in mixtures of 4 fatty acids with BSA.^a

	1:1:1:1				30:35:20:15
	Experiment		Model		Experiment		Model
	FFAu (nM)	X (%)	FFAu (nM)	X (%)	FFAu (nM)	X (%)	FFAu (nM)	X (%)
AA	0.90	29	0.92	30	1.08	34	1.11	35
OA	0.81	26	0.72	23	1.21	39	1.01	32
PA	0.81	26	0.79	26	0.55	17	0.63	20
SA	0.58	19	0.67	22	0.31	10	0.40	13
Total	3.10		3.10		3.15		3.15
AA	7.75	39	7.70	39	9.45	46	9.10	45
OA	3.48	17	3.94	20	4.60	23	5.40	27
PA	5.32	27	4.97	25	4.54	22	3.90	19
SA	3.41	17	3.35	17	1.79	9	1.97	10
Total	20.0		20.0		20.4		20.4

^aExperimental FFA_ui values were determined from two different mixtures (1:1:1:1 and 30:35:20:15) of AA, OA, PA and SA by analyzing the measured probe responses with equation (4). The mixture ratios shown in the first line of the table indicate the relative volume ratios of the FFA:BSA complexes used to prepare the mixtures (Methods) and these are roughly the final FFA_ui ratios observed in the mixtures. Two concentrations of total FFA_u (3.1 and 20 nM) were used for each distribution. Model values of FFA_ui were calculated using the single class model (Equations (A4 & A6)). Also shown is the fraction of each FFA_ui in the mixture (X in %).