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. Author manuscript; available in PMC: 2008 Aug 23.
Published in final edited form as: Biochemistry. 2006 Jan 10;45(1):94–101. doi: 10.1021/bi052015l

Figure 3.

Figure 3

Example of the fluorescence distribution of library members and an example of the mass spectrum obtained for microsequencing of a top hit sequence. Left: A histogram displaying the number of beads versus fluorescence intensity of a portion of the library (the sharp peak on the left corresponds to bubbles trapped in the instrument). Right: A representative mass spectrum of the cleavage products of one of the top forty most fluorescent beads. The amino acids corresponding various mass differences are annotated. Signature doublets are obtained for asparagine and histidine as result of the use of both capping reagents (1 and 2) during those coupling reactions.