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. Author manuscript; available in PMC: 2008 Aug 24.
Published in final edited form as: Biochemistry. 2006 Mar 14;45(10):3396–3403. doi: 10.1021/bi052513u

Figure 5.

Figure 5

Csk does not directly phosphorylate GC-A. A. Coomassie blue staining of purified recombinant Csk, Src and GST-GC-A intra (the intracellular domain of GC-A). B. In vitro kinase assay of Csk using GST-CDB3 and GST-GC-A as exogenous substrates. The in vitro kinase assay was performed in the presence of ATP and the results were analyzed with SDS-PAGE and western blotted with anti-phospho-Tyr antibody. C. In vitro kinase assay of Src using GST-CDB3 and GST-GC-A-intra as substrates. The kinase assay was performed in the presence of γ-32P-ATP and the results were analyzed by SDS-PAGE and autoradiography.