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Methylation-specific PCR (MSP) of OPCML, CTNNG, HOXA9, EMP3, SYK, HSP47(a), HSP47(b) and TMS1 in neuroblastoma and phaeochromocytoma tumours. Bisulphite-modified DNA was amplified with primers specific for unmethylated (U) and methylated (M) DNA. Sample number above lane. NA, normal adrenal DNA. Sizes of the PCR products are indicated by arrows, U for unmethylated and M for methylated respectively. Positive control is Sss1-treated DNA.
