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Actin filament velocity as a function of cMyBP-C concentration, using whole myosin (A), HMM (B), and myosin S1 (C). Cardiac MyBP-C caused a concentration dependent reduction in velocity for myosin and its subfragments. These data demonstrate that the inhibition of velocity by cMyBP-C is not the result binding of cMyBP-C LMM or myosin S2.
