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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: J Mol Cell Cardiol. 2008 Mar 29;44(6):1053–1061. doi: 10.1016/j.yjmcc.2008.03.012

Figure 5.

Figure 5

Co-sedimentation of myosin S1 and cMyBP-C with actin. Representative SDS PAGE (A) and composite densitometric ratios (B) of cMyBP-C (circles) and myosin S1 (squares) cosedimented with actin in the presence of ATP. As is demonstrated the amount of myosin S1 cosedimented with actin is reduced with the addition of cMyBP-C. In contrast, in experiments where ATP was not added (C and D), myosin rigor binding to actin is not affect by cMyBP-C but cMyBP-C binding to actin is reduced nearly 3 fold. These data demonstrate that cMyBP-C competes with myosin S1 for actin binding.