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Immortalization of HPSCs. Primary HPSCs were exposed to lentiviral vectors carrying hTERT and SV40-TAg. Immunohistochemical staining of HPSCs transduced with hTERT and SV40-TAg for αSMA (A) and vimentin (B). C, growth curve for primary HPSCs, HPSCs transduced with SV40-TAg, and HPSCs transduced with both hTERT and SV40-TAg. HPSCs carrying hTERT and Tag show no evidence of senescence at >100 passages.
