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BxPC3 cells were treated with gemcitabine (100 μmol/L) with or without conditioned media from HPSCs (1 μg/μL) for 48 h. A, apoptosis was detected using TUNEL assay and cell nuclei were stained with DAPI. B, percentage of apoptotic cells was determined in 10 random fields for each condition. SFM, serum-free media.
