Table 1.

Physiological parameters in experimental groups

	Control+NS	STZ+NS	STZ+MM	STZ+siRNA
Fasting glucose 0 wk, mg/dl	97.5±3.7	343±35.9‡	388±24.1‡	354±16.9‡
Fasting glucose 7 wk, mg/dl	119.8±3.9	569±28.8‡	566±29.8‡	558±30.7‡
Serum creatinine 7 wk, mg/dl	0.19±0.02	0.43±0.23*	0.41±0.17*	0.40±0.17*
Body wt 7 wk, g	26.25±1.71	19.25±0.96‡	19.75±0.50‡	20.52±0.58‡
Kidney wt 7 wk, g	0.270±0.01	0.212±0.02‡	0.222±0.01‡	0.205±0.01‡
Kidney wt/body wt 7 wk, %	1.03±0.04	1.10±0.13*	1.13±0.07*	1.06±0.05$
Urinary volume 0 wk, ml	2.90±0.57	3.40±0.57	2.75±1.06	2.75±0.35
Proteinuria 0 wk, mg	8.58±1.14	7.07±3.45	7.14±1.35	6.37±0.88
Albuminuria 0 wk, μg	111.6±36.3	250.8±96.7	180.9±38.1	190.8±48.5
Urinary volume 3.5 wk, ml	2.15±0.21	5.1±0.57*	6.6±1.16*	6.15±0.36*
Proteinuria 3.5 wk, mg	12.64±0.80	15.58±2.78	18.01±1.58	16.49±0.32
Albuminuria 3.5 wk, μg	119.2±26.9	293.9±45.5*	246.1±76.5*	193.7±48.3*
Urinary volume 7 wk, ml	2.53±1.37	6.45±0.77*	6.85±1.62*	6.65±1.06*
Proteinuria 7 wk, mg	10.92±4.11	36.59±5.08†	42.07±5.03†	32.07±5.60*§
Albuminuria 7 wk, μg	120.8±77.9	413.4±46.1†	362.2±21.2†	200.6±42.5*§

Values are means ± SE; n = 4/group. DBA/2J mice were injected with streptozotocin (STZ), and 1 wk later injections were commenced, twice weekly for ≤7 wk with either normal saline (STZ+NS), mismatched oligos (STZ+MM), or cholesterol-tagged 12/15-lipoxygenase (12/15-LO) small interfering RNA (siRNA; STZ+siRNA). A control nondiabetic group of DBA/2J mice was injected with normal saline alone (control+NS) Parameters were first measured soon after establishment of hyperglycemia (1 wk post-STZ injection) at 0 wk before oligo delivery (0 wk), 3.5 wk after the first oligo injections (3.5 wk), and 7 wk after the first oligo injections at the end of the experimental period (7 wk).

^*P < 0.05,

^†P < 0.01,

^‡P < 0.001 vs. control+NS.

^§P < 0.05 vs. STZ+MM.