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. 2008 Jun 6;295(2):H499–H508. doi: 10.1152/ajpheart.00374.2008

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Fig. 3. — Representative tracings for the effects of hemoglobin (Hb) on nitrite-induced vasorelaxation in aerobic conditions (pH 7.4, 37°C). Aortic rings were first contracted with phenylephrine (PHE) (10 μM), and Hb (20 μM) was then added after nitrite. A: representative tracing for the effect of Hb on nitrite (100 μM) prerelaxed aortic ring. B: representative tracing for the effect of Hb on nitrite (500 μM) prerelaxed aortic ring. Ordinate, aortic tension expressed in grams (g); abscissa, time in min. Arrows show the time at which different agents were added. Each experiment was repeated at least 5 times, and findings were reproducible as shown.