Fig. 6.

Effects of Hb and ODQ on electrochemical measurements of nitrite-induced NO from aortic rings in aerobic conditions (pH 7.4, 37°C). Nitrite (500 μM) was first added to the organ bath to generate NO from the aortic preparation, and Hb (50 μM) or ODQ (10 μM) was then added at the steady-state level of NO. A: representative tracing for the effect of Hb on nitrite-induced NO in endothelium-intact ring. B: representative tracing for the effect of Hb on nitrite-induce NO in an endothelium-denuded ring. C: representative tracing for the effect of ODQ on nitrite-induced NO in endothelium-intact ring. Ordinate, NO concentration in nM; abscissa, time in s. Arrows show the time at which different drugs were added. Findings were reproducible with each experiment, n ≥ 4.