Table 1.
Extracellular (bath) and intracellular (pipette) solutions used in study
| Pipette Solution INaT, INaL |
Bath Solutions |
||
|---|---|---|---|
| INaT for VCs | INaL for VCs, INaT for αHEK | ||
| NaCl | 5 | 5 | 140 |
| KCl | 5.4 | ||
| CsCl | 133 | 133 | 5.4 |
| CaCl2 | 1.8 | 1.8 | |
| MgCl2 | 2 | 2 | |
| MgATP | 2 | ||
| TEA | 20 | ||
| Nifedipine | 0.002 | 0.002 | |
| EGTA | 10 | ||
| HEPES | 5 | 5 | 5 |
| pH | 7.3 CsOH | 7.3 NaOH | 7.3 NaOH |
Concentrations are expressed in mM. INaT, peak transient Na+ current; INaL, late Na+ current; VCs, ventricular cardiomyocytes; αHEK, HEK293 cell line stably expressing Nav1.5 or Nav1.2; TEA, tetraethylammonium.