Table 1.
Oligonucleotides for real-time RT-PCR
| mRNA Targets | Species | Description (Alternative Name) |
Primers |
|
|---|---|---|---|---|
| Sense | Antisense | |||
| BMP-4 | Rat | Bone morphogenetic protein-4 | 5′-GAATAAGAACTGCCGTCG-3′ | 5′-CCTTGTCGTACTCGTCC-3′ |
| BMP-2 | Rat | Bone morphogenetic protein-2 | 5′-TCAAGCCAAACACAAACAG-3′ | 5′-CGCTAAGCTCAGTGGG-3′ |
| BMPR1a | Rat | Bone morphogenetic protein receptor, type IA (ALK3) | 5′-GCTTCAGACATAGGACTTCAG-3′ | 5′-TGGACAGGATAGTGAGTAACC-3′ |
| BMPR1b | Rat | Bone morphogenetic protein receptor, type IB | 5′-AGAAGCATCCTTAGCCCAAG-3′ | 5′-GGTTCCTCTGTGTCTGTCC-3′ |
| BMPR2 | Rat | Bone morphogenetic protein receptor, type II | 5′-CCAGTCCTGATGAACATGAACC-3′ | 5′-TTCCCTCCTGTCTACCAAACG-3′ |
| ACVR2a | Rat | Activin A receptor, type IIA | 5′-GCTTGTGAATGTGTAGTGTGC-3′ | 5′-GCAACCGTGGAACTGAGG-3′ |
| ACVR2b | Rat | Activin A receptor, type IIB | 5′-ATCAGGAGGTCGGTCAACAG-3′ | 5′-TGGGCTTAGATGCTGGACTC-3′ |
| ACVR1 | Rat | Activin A receptor, type I (ALK2) | 5′-AAGACAGCGGCAAGTTGAATC-3′ | 5′-AAGGGACGCAGCAACAGAC-3′ |
| ACVRL1 | Rat | Activin A receptor type II-like 1 (ALK1) | 5′-CCGAAGCCTGAGTTGATGGAC-3′ | 5′-GCCCGAGCCCTACTTTGC-3′ |
| CHRD | Rat | Chordin | 5′-TACACATTGCCACTTACAC-3′ | 5′-CCTCTGAGCCATAGAATCC-3′ |
| NOG | Rat | Noggin | 5′-GCCAGCACTATCTACACATCC-3′ | 5′-CAGCAGCGTCTCGTTCAG-3′ |
| SMAD-1 | Rat | Mothers against decapentaplegic homolog 1 (MADH1) | 5′-CTGTCTCATCCGCTCCAC-3′ | 5′-GTCTGTATTGTAAACGCCATAAG-3′ |
| SMAD-4 | Rat | 5′-CACTGGTTGTTATGTATGAAGGAG-3′ | 5′-ACAGGTGACTTAACTTTGAGAAC-3′ | |
| SMAD-5 | Rat | 5′-CAGCACATATCCCAACTC-3′ | 5′-GGCATGATCTGAGGAATC-3′ | |
| gp91phox | Rat | Cytochrome b-245, β-polypeptide (NOX2) | 5′-GGATGAATCTCAGGCCAA-3′ | 5′-TTAGCCAAGGCTTCGG-3′ |
| p47phox | Rat | Neutrophil cytosolic factor 1 (NCF-1), NADPH oxidase organizer 2 (NOXO2) | 5′-AGCACCAAGAGGAAACT-3′ | 5′-CCTAGCAATACCCGTGGA-3′ |
| p22phox | Rat | Cytochrome b-245, α-polypeptide (CYBA) | 5′-TGCCAGTGTGATCTACC-3′ | 5′-AGCTATTAACCATGTTTATTACAGT-3′ |
| NOX1 | Rat | NADPH oxidase homolog 1 | 5′-TGAATCTTGCTGGTTGACACTTGC-3′ | 5′-GAGGGACAGGTGGGAGGGAAG-3′ |
| NOX4 | Rat | Renox | 5′-TGCCTCCATCAAGCCAAG-3′ | 5′-TTCCAGTCATCCAGTAGAGTG-3′ |
| HPRT | Rat | Hypoxanthine phosphoribosyltransferase 1 (housekeeping gene) | 5′-AAGACAGCGGCAAGTTGAATC-3′ | 5′-AAGGGACGCAGCAACAGAC-3′ |
| ICAM-1 | Human | Intercellular adhesion molecule 1 | 5′-CTCTCGCTCTGTCACC-3′ | 5′-GGAAGTCTGGGCAATGT-3′ |
| Id1 | Human | DNA-binding protein inhibitor | 5′-CGCATCTTGTGTCGCTGAAG-3′ | 5′-GGGAGACCCACAGAGCAC-3′ |
| HPRT | Human | Hypoxanthine phosphoribosyltransferase 1 (housekeeping gene) | 5′-AGATGGTCAAGGTCGCAAG-3′ | 5′-TTCATTATAGTCAAGGGCATATCC-3′ |