Figure 3.

mcd4–174 Cells exhibit morphological aberrancies at nonpermissive temperature. Wild-type (SEY6210) and mcd4–174 (GY1450) cells were incubated at 24 or 38°C for 3 h and prepared for electron microscopy. Wild-type cells at 38°C are shown in panel A. mcd4–174 Cells at 24°C are shown in panel B. mcd4–174 Cells at 38°C are shown in panels C–F. The nucleus (N), vacuole (v), and mitochondria (m) are indicated. Arrows point to accumulated membranes likely to correspond to ER. Arrowheads point to other accumulated membranes, which may also represent ER. Panel G is a higher magnification photograph of the accumulated membranes observed in the cell shown in panel C. Bar, 0.5 μm.