mcd4–174 Is not defective for transport or
modification of CPY and HSP150. Wild-type (WT; GY1446) and
mcd4–174 (GY1450) cells were incubated at 38°C for 15
min, pulse-labeled with 35S-methionine for 10 min, chased
for 30 min, separated into intracellular (C) and extracellular/media
(M) fractions, and processed for immunoprecipitation with antisera to
CPY (A) or HSP150 (B). In panel A, the positions of p1 (ER), p2
(Golgi), and m (mature/vacuolar) CPY are indicated, and only the
intracellular fraction is shown (no CPY was found in the extracellular
[M] fraction).