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. 1999 Mar;10(3):627–648. doi: 10.1091/mbc.10.3.627

Figure 4.

Figure 4

mcd4–174 Is not defective for transport or modification of CPY and HSP150. Wild-type (WT; GY1446) and mcd4–174 (GY1450) cells were incubated at 38°C for 15 min, pulse-labeled with 35S-methionine for 10 min, chased for 30 min, separated into intracellular (C) and extracellular/media (M) fractions, and processed for immunoprecipitation with antisera to CPY (A) or HSP150 (B). In panel A, the positions of p1 (ER), p2 (Golgi), and m (mature/vacuolar) CPY are indicated, and only the intracellular fraction is shown (no CPY was found in the extracellular [M] fraction).