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mcd4–174 Cells are defective for incorporation of inositol into proteins. Wild-type (WT; GY1446), sec18–1 (SEY5188), and mcd4–174 (GY1450) cells were incubated in inositol-free medium at 38°C for 15 min and then labeled with myo-[2-[3H]]inositol for 45 min; ∼1 OD-eq of the total protein extract for each sample was resolved by SDS-PAGE. Positions of molecular mass markers are shown to the right.
