Figure 8.

IPC synthesis in mcd4–174. Wild-type (WT; GY1446), sec18–1 (SEY5188), and mcd4–174 (GY1450) cells were incubated in inositol-free medium at 38°C for 15 min, labeled with myo-[2-[³H]]inositol for 20 min, and chased with cold myo-inositol for 60 min. Lipids were extracted and ∼0.5 OD-eq per sample were resolved by TLC using solvent 1. The positions of PI, IPCs, MIPC, lyso-PI (lyso-phosphatidylinositol), M(IP)₂C, and the origin are shown to the right. ** Denotes a lipid that accumulates in mcd4–174 but not wild-type or sec18–1 cells, and the IPCs; * Indicates that IPCs may mask another lipid species accumulating in mcd4–174.