Fig. 5.
Phosphorylation levels of Tm at Ser283 are increased. Neonatal and adult Tm in-gel digests (∼2 μg each) were subjected to TiO2 Nutip enrichment and LTQ-FT analysis. A: representative ECD-MS2 spectrum. The phosphorylation site was determined to be at Ser283. B: Western blots using antibodies specific to phospho-Ser283 and total Tm confirmed increased levels of phosphorylation of Tm at Ser283 in neonatal samples.