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Effect of HS on activation of AKT in NRVM. NRVM were subjected to HS or control conditions. Cell lysates were probed for phosphorylated (p-)AKTser473 to detect activated AKT. Compared with control myocytes, myocytes subjected to HS showed increased phosphorylation/activation of AKT (*P < 0.007, C vs. HS; **P < 0.05, 10'CI vs. HS+10'CI; n = 3). y-Axis data are plotted as fraction of control myocyte levels. The amount of total AKT present in each sample was not significantly different.
