Fig. 8.

Effect of HS on integrin-linked kinase (ILK) activity. NRVM were infected with FRNK virus (inhibitor of FAK) or empty virus (SR) for 48 h. Another group of myocytes were subjected to HS. In a separate control experiment (n = 1), control myocytes were treated with 100 ng/ml insulin-like growth factor for 15 min as a positive control for ILK activity. Myocyte extracts were immunoprecipitated with anti-ILK antibodies, followed by an in vitro kinase assay using GSK-3 fusion protein as a substrate (see materials and methodsfor details). Activation of ILK was measured by probing for anti-phospho-GSK-3α/β (Ser 21/9). Total ILK protein present in each lane was detected with anti-ILK antibody after stripping the blot. The graph shows that neither HS nor FRNK infection resulted in increased ILK activity. y-Axis data are plotted as arbitrary units of density. Total ILK present was not different between groups. Representative blot is shown. P = NS, HS vs. C and FRNK vs. SR; n = 3.