TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Characteristic(s)a | Source or reference |
|---|---|---|
| Strains | ||
| E. coli DH5α | Wild type | Invitrogen |
| P. putida S12 | Wild type | Hartmans et al. (9) |
| P. putida S12xylAB | P. putida S12 containing plasmid pJTxylAB | This study |
| P. putida S12xylAB_FGH | P. putida S12 containing plasmid pJTxylAB_FGH | This study |
| P. putida S12xylAB2 | P. putida S12 containing plasmid pJTxylAB, evolved to efficient pentose utilizer | This study |
| P. putida S12xylAB2c | P. putida S12xylAB2 cured from pJTxylAB | This study |
| P. putida S12Δgcd | P. putida S12 glucose dehydrogenase knockout | This study |
| P. putida S12Δgcd_xylAB | P. putida S12 glucose dehydrogenase knockout containing plasmid pJTxylAB | This study |
| P. putida S12araFGH | P. putida S12 containing plasmid pBTaraFGH | This study |
| P. putida S12xylAB_araFGH | P. putida S12 containing plasmids pJTxylAB and pBTaraFGH | This study |
| Plasmids | ||
| pJNTmcs(t) | Apr Gmr; basic expression vector derived from plasmid pJWB1 (28) containing the salicylate-inducible promoter nagR-nagAa | Unpublished data |
| pJTTmcs | Apr Gmr; expression vector containing the constitutive tac promoter, derived from pJWB1 | Unpublished data |
| pJTmcs | Apr Gmr; expression vector containing the constitutive tac promoter without the tac RBS | This study |
| pBTmcs | Cmr; expression vector containing the constitutive tac promoter without the tac RBS | Unpublished data |
| pJTxylAB | pJTmcs containing the xylAB genes from E. coli DH5α | This study |
| pJTxylAB_FGH | pJTmcs containing the xylAB and xylFGH genes from E. coli DH5α | This study |
| pBTaraFGH | pBTmcs containing the araFGH genes from E. coli DH5α | This study |
| pJQ200SK | P15A ori sacB RP4 Gmr(pBluescriptSK); suicide vector | Quandt et al. (20) |
| pJQgcd::Kana | pJQ200SK containing a Kmr-disrupted copy of the gcd gene | This study |
a
Apr, ampicillin resistance; Gmr, gentamicin resistance; Cmr, chloramphenicol resistance; Kmr, kanamycin resistance; ori, origin of replication; sacB, gene producing levansucrase.