TABLE 2.
Oligonucleotide primers used in this study
| Primer | Target | Sequence (5′ → 3′)a | Characteristic |
|---|---|---|---|
| Primer 1 | xylA from E. coli | GCGGCGGGTACCATGCAAGCCTATTTTGACC | KpnI cohesive end |
| Primer 2 | xylB from E. coli | GCGGCGGCGGCCGCTTACGCCATTAATGGCAG | NotI cohesive end |
| Primer 3 | 5′ End of gcd | GCGGCGGCCGCTTACTCAGCTAATTTGTAAGCGAT | NotI cohesive end |
| Primer 4 | Positions 1154-1134 in gcd | GCGTCTAGACCAACATGTGGTCGATCGCCA | XbaI cohesive end |
| Primer 5 | Positions 1261-1281 in gcd | GCGTCTAGAGATCACCCCGGACGGCTCATT | XbaI cohesive end |
| Primer 6 | 3′ End of gcd | GCGGGATCCATGAGCACTGAAGGTGCGAACC | BamHI cohesive end |
| Primer 7 | 5′ End of loxP-Kmr-loxP | GCTCTAGAATAACTTCGTATAATGTATGCTATAC | XbaI cohesive end |
| Primer 8 | 3′ End of loxP-Kmr-loxP | CGCGCAATTAACCCTCACT | |
| Primer 9 | Positions 2088-2106 in gcd | ACGGTAGCAGCAGTACCAC | qPCR primer |
| Primer 10 | Positions 2266-2285 in gcd | TACTACCTGATCGCCGGTAT | qPCR primer |
| Primer 11 | Positions 588-608 in gcd | AATGCGCCAGGCCTCTTCCAG | Sequencing primer |
| Primer 12 | Positions 397-376 upstream of gcd | CGCCACCGTGCATGACAAGAAG | Sequencing primer |
a
The restriction sites used for cloning are underlined.