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. 2008 May 23;74(16):5250–5253. doi: 10.1128/AEM.00272-08

FIG. 1.

FIG. 1.

TLC analysis of the 6NSE product. (A) TLC analysis of the 6NSE products of various cell fractions or resting cells of G. oxydans M5 catalyzing the 1NSL reaction. Lanes: 1, resting cell reaction mixture; 2, membrane fraction reaction mixture; 3, soluble fraction reaction mixture; 4, purified enzyme reaction mixture. (B) TLC analysis of the 6NSE product of various mutant resting cells catalyzing the 1NSL reaction. Lanes 1, wild-type G. oxydans M5 reaction mixture; 2, G. oxydans M6 defective sldA reaction mixture; 3, G. oxydans M7 complementation of the sldAB genes reaction mixture; 4, standard of substrate 1NSL.