TABLE 1.
Bacterial strains and plasmids used
| Strain or plasmid | Relevant characteristic(s)a | Reference or source |
|---|---|---|
| Strains | ||
| G. oxydans M5 | Wild strain producing 6NSE from 1NSL | Our laboratory |
| G. oxydans M6 | G. oxydans M5 derivative; sldA::kan | This study |
| G. oxydans M7 | G. oxydans M6 containing pBBR1MCS5-sldAB | This study |
| E. coli JM109 | recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 Δ(lac-proAB)/F′ [traD36 proAB+lacIqlacZ::M15] | |
| BW25113 | lacIqrrnBT14 ΔlacZwj16hsdR514 ΔaraBADAH33 ΔrhaBADLD78 | 3 |
| Plasmids | ||
| pKD46 | Red recombinase expression plasmid; Ampr | 3 |
| pKD46RCm | pKD46 derivative; Cmr; red recombinase expression plasmid | Our laboratory |
| pKD4 | Knr gene cassette | 3 |
| pGEM-sldAB | pGEM-T carrying sldAB; Ampr | This study |
| pGEM-sldB-sldA::kan | pGEM-sldAB carrying a modified sldA gene; Ampr Knr | This study |
| pBBR1MCS-5 | Broad-host-range cloning vector; Gmr | 10 |
| pBBR1MCS5-sldAB | pBBR1MCS-5 carrying sldAB; Gmr | This study |
a
Cmr, chloramphenicol resistant; Knr, kanamycin resistant; Ampr, ampicillin resistant; Gmr, gentamicin resistant; cat, chloramphenicol resistance gene; kan, kanamycin resistance gene.