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Purification of 1NSL dehydrogenase of G. oxydans M5
| Purification step | Total activity (units)a | Total protein (mg) | Sp act (U/mg of protein)a | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|
| Membrane purification | 2,016 | 1,588 | 1.3 | 100 | 1 |
| Solubilization | 1,360 | 108 | 13 | 67 | 10 |
| CM-cellulose chromatography | 1,172 | 31 | 38 | 58 | 30 |
| Sephacryl HR 400 chromatography | 589 | 9.4 | 62 | 29 | 49 |
Standard deviations of the determination of specific enzyme activities were less than 20%.
