FIG. 2.

Cotranscription of queA with arcR and primer extension analysis of S. gordonii queA. (A) Transcription initiation site of S. gordonii queA analyzed by primer extension. The arrow indicates the transcription initiation site at a G residue 181 bases upstream of the queA start codon. (B) RT-PCR analysis of mRNA from S. gordonii grown in TY medium containing 10 mM galactose with 50 mM arginine. Primers specific for the arcR and queA intergenic regions were used to amplify cDNA. Lane 1, negative control, in which the reaction mixture contained mRNA from S. gordonii but no RT was performed; lane 2, positive control, in which the control RNA from the RT reaction kit was used as the template to perform RT-PCR as recommended by the supplier; lanes 3 and 4, arcR-queA intergenic region, in which mRNA from S. gordonii and chromosomal DNA, respectively, were used as the templates for RT-PCR.