FIG. 5.

Quantitative real-time PCR of arcA gene expression. After RT from 1 μg of total RNA from wild-type, QueA-deficient, and CcpA-deficient strains of S. gordonii, the amount of arcA gene cDNA was determined by real-time PCR using SYBR green. The data are means ± standard deviations, which were obtained using three different RNA preparations and RT reactions. An asterisk indicates that the copy number of the arcA mRNA of the QueA-deficient or CcpA-deficient strain differs significantly from that of the wild-type strain of S. gordonii (P < 0.05, as determined by the Student t test).