FIG. 1.

The pH values (×) and cell numbers of samples from mine tailing dumps located in Selebi-Phikwe, Botswana (A and B); Kristineberg, Sweden (C and D); and Freiberg, Germany (E and F). The cell numbers were detected by Sybr green II direct counting (•), CARD-FISH for Bacteria ( ), the MPN technique for detection of Fe(II) oxidizers (○), Q-PCR for Bacteria (▵), and Q-PCR for Archaea (□). The number of Archaea detected by CARD-FISH was below the detection limit of 10⁵ cells g⁻¹ dry weight (dw) at all three sites. Note different zones and depth scales. (Panels A and B are both adapted from references 30 and 55 with permission from Elsevier. Panel C is adapted from reference 25 with permission of the publisher [copyright 2008 American Chemical Society] and reference 30 with permission from Elsevier. Panel D is adapted from reference 30 with permission from Elsevier. Panels E and F are both adapted from reference 31 with permission and reference 21 with permission from Elsevier.)