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FISH, CARD-FISH, and DAPI (4′,6′-diamidino-2-phenylindole) fluorescence images for a selected sample from the oxidation zone of the sulfidic mine waste dump in Freiberg, Germany. (A and C) Weak FISH (A) and bright CARD-FISH (C) signals of cells detected with probes EUB338 I to III specific for Bacteria. (B and D) Same microscopic fields as panels A and C, respectively, with UV excitation (DAPI staining).
