TABLE 1.
Primers used for RT-PCR cloning of hypothemycin cDNAs
| Primer | Sequence | Product (size of product [kb]) |
|---|---|---|
| P450-F(NdeI) | CCATATGGCAGTGTTACCCCAAG | P450 (1.5) |
| P450-B | TAGATGGCAGTCGAGACTACC | |
| GST-F(NdeI) | GCATATGGTTATCAAGCTGTACGGCTC | GST (0.7) |
| GST-B | AAACTTCATGTACAATCTTTCAGCTTC | |
| NR-PKS-F(NdeI) | CTCATATGGTGACTGTACCACAGAC | NR-PKS A (1.7) |
| NR-PKS-B1704 | CCAGGAGTGAGGATACCCG | |
| NR-PKS-F1239 | CGACATCAATGAGTTCTACTGTACC | NR-PKS B (1.6) |
| NR-PKS-B2848 | AGCGTGACAACAGCGAGCTG | |
| NR-PKS-F2691 | CGGGACAGGGCTCTCACTAC | NR-PKS C (1.8) |
| NR-PKS-B4441 | CGCTCTCACCCCAGTAAGG | |
| NR-PKS-F4239 | ATACTTCGTCAAGGCTCGTATG | NR-PKS D (2.0) |
| NR-PKS-B6238 | CAAATCGTCTCCCAAAAAGATACTC | |
| OMT-F(NdeI) | CCATATGAGTGCATCCAATGGCGAG | OMT (1.2) |
| OMT-B | GGTACTCCCGTCATCGTTGGG | |
| FMO-F(NcoI) | CCATGGCGAGAGCAATACTGCCAGAC | FMO (1.9) |
| FMO-B1897 | CGAGGGCTTCAAGGATAGG | |
| R-PKS-F(NdeI) | CCATATGCCTTCTACCAGCAATCC | R-PKS A (1.9) |
| R-PKS-B1777 | CTTGGAAAGCTCAACGATAGG | |
| R-PKS-F1782 | GCTGTGAATGGGACCCTATC | R-PKS B (2.8) |
| R-PKS-B4840 | TGCTCAAGCTCCAACAGAGAAATG | |
| R-PKS-F3743 | TTCCGAGCTTGATATGGATTC | R-PKS C (1.7) |
| R-PKS-B5448 | CACGGGAGTTGAAGATGTGC | |
| R-PKS-F5154 | CGTGTTTGCACCATGAACGTC | R-PKS D (2.1) |
| R-PKS-B7523 | CCCTCCGTAAACGCCTTAAACAG |