TABLE 3.
Conversion of RAL substrates by OMT or FMO in vitroa
| Enzyme | Substrate | Substrate peak retention time (min) | Retention time(s) of postreaction peak(s) (min) (area) | % Conversion |
|---|---|---|---|---|
| OMT | 4-O-Desmethylhypothemycin | 3.50 | 5.26 (1,212) | 100 |
| Zearalenone | 8.14 | 11.4 (310) | 100 | |
| 1′,2′-Epoxyaigialomycin D | 3.44 | 3.44 (31), 5.49 (1,354) | 98 | |
| FMO | Desepoxyhypothemycin | 5.95 | 5.26 (446), 5.95 (74) | 86 |
| Zearalenone | 8.14 | 7.02 (65), 8.14 (30) | 68 |
a
Peaks having the characteristic RAL UV absorbance profile are shown along with their retention times and peak areas at 267 nm. Reactions were performed as described in Materials and Methods, and the reaction mixtures were incubated for 60 min.