TABLE 1.

Oligonucleotide sequences and PCR amplification conditions used to amplify EspA and EspB genes and the γ-intimin_1-280 gene fragment from EHEC strains

Gene or gene fragment	GenBank accession no.	Primer	Oligonucleotide sequence (5′-3′)	Positions	Amplification conditions
EspB	U65681	espBup2	GGATCCATGAATACTATTGATTATAC	1-945	94°C, 2 min (1 cycle); 94°C, 1 min; 50°C, 1 min; 72°C, 1 min (30 cycles)
		espBrev	AAGCTTTTAACCAGCTAAGCGAACCG
EspA	AY223511	espAfor	GGATCCATGGATACATCAACTGCAACATC	1-579	94°C, 2 min (1 cycle); 94°C, 1 min; 55°C, 1 min; 72°C, 1 min (30 cycles)
		espArev	AAGCTTTTATTTACCAAGGGATATTGCTG
γ-Intimin1-280	Z11541	ctereaeup	GGATCCCAAACCAAGGCCAGCATTAC	1962-2804	94°C, 1 min; 53°C, 1 min; 72°C, 1 min (30 cycles)
		Grev	AAGCTTTTATTCTACACAAACCGCATAGA