TABLE 2.
Host ranges and transformation frequencies of pBC1-derived vectors
| Species and strain | No. of transformants per μg of DNAb
|
||
|---|---|---|---|
| pAM1 | pAM2a | pAM4 | |
| B. adolescentis LMG 10502 | — | — | 9.2 × 102 |
| B. animalis subsp. animalis LMG 10508 | — | — | 4.0 × 101 |
| B. animalis subsp. lactis Bb12 | — | — | 1.6 × 102 |
| B. breve LMG 13208 | — | — | 1.0 × 102 |
| B. breve UCC 2003 | 2.3 × 102 | 6.4 × 104 | 1.4 × 102 |
| B. dentium F101 | — | — | 9.5 × 101 |
| B. longum L25 | — | — | 6.6 × 101 |
| B. pseudolongum subsp. pseudolongum LMG 11571 | — | — | 6.3 × 101 |
| B. pseudocatenulatum M115 | 2.5 × 105 | 8.3 × 105 | 1.0 × 105 |
| B. thermophilus LMG 11571 | — | — | 4.6 × 101 |
| Corynebacterium glutamicum LMG 19741 | — | — | 3.0 × 100 |
| Enterococcus durans L72 | — | — | 0 |
| Lactobacillus casei ATCC 393 | — | — | 0 |
| Lactococcus lactis subsp. cremoris MG 1363 | 0 | 0 | 0 |
| Lactococcus lactis subsp. lactis IL-1403 | — | 0 | 0 |
| Pediococcus acidilactici LMG 11384 | — | — | 0 |
| Escherichia coli DH5α | — | 0 | — |
a
Whenever possible, pAM2 DNA was isolated from the same strain to which it was transformed.
b
—, not done or not applicable.