FIG. 2.

Role of proteins in B. bifidum MIMBb75 adhesion to Caco-2 cells. (a) Percent changes in adhesion after incubation of MIMBb75 cells in a proteinase K (Prot. K) solution and after extraction of surface proteins with 5 M LiCl. *, statistically significant difference compared to untreated cells (P < 0.05). (b) SDS-PAGE analysis of MIMBb75 cell wall-associated proteins grown under standard conditions (lane A) and after treatment with 5 M LiCl (lane B). The molecular masses (in kDa) of the standard proteins are indicated at right.