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. 2008 Jun 6;74(15):4853–4866. doi: 10.1128/AEM.02756-07

TABLE 5.

Comparisons of recovery rates for stress-exposed Salmonella serovar Enteritidis, E. coli O157:H7, and L. monocytogenes cells in TSBYE, SEL, and the respective individual selective enrichment brothsa

Stress or condition Organism Log10 CFU/ml (mean ± SD) at 0 h Log10 CFU/ml (mean ± SD) at 3 h in:
Log10 CFU/ml (mean ± SD) at 6 h in:
TSBYE Selective enrichment brothb SEL TSBYE Selective enrichment brothb SEL
Temp (°C) Salmonella serovar
    37     Enteritidis 7.27 ± 0.24 8.81 ± 0.20* 8.35 ± 0.15 8.29 ± 0.28 9.23 ± 0.10* 8.41 ± 0.29 9.27 ± 0.14
    4 6.25 ± 0.14 8.31 ± 0.34* 7.35 ± 0.48* 7.66 ± 0.22* 9.10 ± 0.05* 8.40 ± 0.05* 9.21 ± 0.14*
pH
    4.5 5.20 ± 0.54 7.22 ± 0.02* 5.39 ± 0.68 5.07 ± 0.00 7.33 ± 0.07* 5.83 ± 1.05 4.89 ± 1.06
    5.5 6.69 ± 0.02 8.71 ± 0.26* 8.25 ± 0.36* 8.28 ± 0.28* 9.05 ± 0.06* 8.53 ± 0.09* 9.35 ± 0.17*
Temp (°C) E. coli O157:H7
    37 7.16. ± 0.11 8.63 ± 0.11* 7.53 ± 0.10 7.84 ± 0.07 9.02 ± 0.11* 8.08 ± 0.18 8.73 ± 0.17
    4 5.57 ± 0.09 8.02 ± 0.14* 5.02 ± 0.73 5.77 ± 0.91 8.86 ± 0.07* 4.90 ± 0.49 7.80 ± 0.23*
pH
    4.5 5.08 ± 0.36 6.43 ± 0.11* 3.21 ± 0.28 3.54 ± 0.28 8.56 ± 0.49* 3.66 ± 0.68 5.24 ± 0.37
    5.5 6.69 ± 0.15 8.43 ± 0.11* 6.89 ± 0.13 7.43 ± 0.21 9.02 ± 0.13* 6.99 ± 0.60 8.66 ± 0.20*
Temp (°C) L. monocytogenes
    37 7.56 ± 0.04 8.72 ± 0.03* 8.20 ± 0.10 8.38 ± 0.12 9.60 ± 0.10* 8.78 ± 0.10 9.17 ± 0.16
    4 6.57 ± 0.08 8.04 ± 0.08* 7.28 ± 0.10 7.67 ± 0.08* 9.06 ± 0.10* 8.36 ± 0.07* 8.88 ± 0.09*
pH
    4.5 6.42 ± 0.17 7.60 ± 0.18* 6.74 ± 0.08 7.33 ± 0.11* 9.22 ± 0.49* 7.75 ± 0.09* 8.06 ± 0.64*
    5.5 6.71 ± 0.04 8.23 ± 0.10* 7.46 ± 0.10 7.91 ± 0.16* 9.56 ± 0.03* 8.41 ± 0.06* 8.99 ± 0.09*
a

Each culture was inoculated at 3 × 102 CFU/ml. Values marked with * indicate the recovery of stressed cells as defined by an increase in cell numbers of ≥1 log in the given medium compared to the initial numbers after stress.

b

The selective enrichment broths were RV broth for Salmonella serovar Enteritidis, mEC+n for E. coli, and FB for L. monocytogenes.