TABLE 5.
Stress or condition | Organism | Log10 CFU/ml (mean ± SD) at 0 h | Log10 CFU/ml (mean ± SD) at 3 h in:
|
Log10 CFU/ml (mean ± SD) at 6 h in:
|
||||
---|---|---|---|---|---|---|---|---|
TSBYE | Selective enrichment brothb | SEL | TSBYE | Selective enrichment brothb | SEL | |||
Temp (°C) | Salmonella serovar | |||||||
37 | Enteritidis | 7.27 ± 0.24 | 8.81 ± 0.20* | 8.35 ± 0.15 | 8.29 ± 0.28 | 9.23 ± 0.10* | 8.41 ± 0.29 | 9.27 ± 0.14 |
4 | 6.25 ± 0.14 | 8.31 ± 0.34* | 7.35 ± 0.48* | 7.66 ± 0.22* | 9.10 ± 0.05* | 8.40 ± 0.05* | 9.21 ± 0.14* | |
pH | ||||||||
4.5 | 5.20 ± 0.54 | 7.22 ± 0.02* | 5.39 ± 0.68 | 5.07 ± 0.00 | 7.33 ± 0.07* | 5.83 ± 1.05 | 4.89 ± 1.06 | |
5.5 | 6.69 ± 0.02 | 8.71 ± 0.26* | 8.25 ± 0.36* | 8.28 ± 0.28* | 9.05 ± 0.06* | 8.53 ± 0.09* | 9.35 ± 0.17* | |
Temp (°C) | E. coli O157:H7 | |||||||
37 | 7.16. ± 0.11 | 8.63 ± 0.11* | 7.53 ± 0.10 | 7.84 ± 0.07 | 9.02 ± 0.11* | 8.08 ± 0.18 | 8.73 ± 0.17 | |
4 | 5.57 ± 0.09 | 8.02 ± 0.14* | 5.02 ± 0.73 | 5.77 ± 0.91 | 8.86 ± 0.07* | 4.90 ± 0.49 | 7.80 ± 0.23* | |
pH | ||||||||
4.5 | 5.08 ± 0.36 | 6.43 ± 0.11* | 3.21 ± 0.28 | 3.54 ± 0.28 | 8.56 ± 0.49* | 3.66 ± 0.68 | 5.24 ± 0.37 | |
5.5 | 6.69 ± 0.15 | 8.43 ± 0.11* | 6.89 ± 0.13 | 7.43 ± 0.21 | 9.02 ± 0.13* | 6.99 ± 0.60 | 8.66 ± 0.20* | |
Temp (°C) | L. monocytogenes | |||||||
37 | 7.56 ± 0.04 | 8.72 ± 0.03* | 8.20 ± 0.10 | 8.38 ± 0.12 | 9.60 ± 0.10* | 8.78 ± 0.10 | 9.17 ± 0.16 | |
4 | 6.57 ± 0.08 | 8.04 ± 0.08* | 7.28 ± 0.10 | 7.67 ± 0.08* | 9.06 ± 0.10* | 8.36 ± 0.07* | 8.88 ± 0.09* | |
pH | ||||||||
4.5 | 6.42 ± 0.17 | 7.60 ± 0.18* | 6.74 ± 0.08 | 7.33 ± 0.11* | 9.22 ± 0.49* | 7.75 ± 0.09* | 8.06 ± 0.64* | |
5.5 | 6.71 ± 0.04 | 8.23 ± 0.10* | 7.46 ± 0.10 | 7.91 ± 0.16* | 9.56 ± 0.03* | 8.41 ± 0.06* | 8.99 ± 0.09* |
Each culture was inoculated at 3 × 102 CFU/ml. Values marked with * indicate the recovery of stressed cells as defined by an increase in cell numbers of ≥1 log in the given medium compared to the initial numbers after stress.
The selective enrichment broths were RV broth for Salmonella serovar Enteritidis, mEC+n for E. coli, and FB for L. monocytogenes.